Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of cellulose reducing ends and primary hydroxyl groups modifications on cellulose-cellulase interactions and cellulose hydrolysis.

Biotechnology and bioengineering·2024
Same author

Rewiring metabolism of Clostridium thermocellum for consolidated bioprocessing of lignocellulosic biomass poplar to produce short-chain esters.

Bioresource technology·2023
Same author

Prospects of thermotolerant Kluyveromyces marxianus for high solids ethanol fermentation of lignocellulosic biomass.

Biotechnology for biofuels and bioproducts·2022
Same author

Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion.

Bioresource technology·2021
Same author

Elucidation of native California Agave americana and Agave deserti biofuel potential: Compositional analysis.

PloS one·2021
Same author

Polyurethanes Based on Unmodified and Refined Technical Lignins: Correlation between Molecular Structure and Material Properties.

Biomacromolecules·2021

Related Experiment Video

Updated: Jun 8, 2026

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates
10:46

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates

Published on: March 12, 2010

Small-scale and automatable high-throughput compositional analysis of biomass.

Jaclyn D DeMartini1, Michael H Studer, Charles E Wyman

  • 1Center for Environmental Research and Technology, University of California Riverside, 92507, USA.

Biotechnology and Bioengineering
|September 11, 2010
PubMed
Summary

A new downscaled biomass analysis method uses 100x less material for faster, high-throughput screening. This method accurately measures carbohydrate composition, supporting biomass research and development.

More Related Videos

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part I: Lignin
12:04

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part I: Lignin

Published on: March 11, 2010

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
11:31

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates
10:46

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates

Published on: March 12, 2010

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part I: Lignin
12:04

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part I: Lignin

Published on: March 11, 2010

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
11:31

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

Area of Science:

  • Biomass analysis
  • Biochemical engineering
  • Analytical chemistry

Background:

  • Conventional biomass composition analysis is resource-intensive.
  • Limited biomass availability hinders high-throughput screening.
  • Need for faster, smaller-scale analytical methods.

Purpose of the Study:

  • Develop a downscaled biomass compositional analysis method.
  • Enable high-throughput pretreatment and hydrolysis (HTPH) screening.
  • Reduce material requirements and analysis time.

Main Methods:

  • Adapted conventional wet chemistry techniques.
  • Scaled down analysis by a factor of 100.
  • Utilized high-performance liquid chromatography (HPLC) vials for analysis.
  • Automated procedure to minimize operator input.

Main Results:

  • Downscaled method uses significantly less biomass material.
  • Statistically identical carbohydrate composition results compared to conventional methods.
  • Provided reasonable estimates for lignin and ash content.
  • Demonstrated validity for HTPH screening studies.

Conclusions:

  • The downscaled method is a valid approach for biomass compositional analysis.
  • Enables accurate calculation of sugar yields.
  • Supports the determination of sugar release trends in HTPH screening.