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Mesothelium of Reissner's membrane in guinea pigs: an electron microscopic study
1Department B. University of Copenhagen, Panum Institute, N. Denmark.
Abstract:
The mesothelial cells of Reissner's membrane in guinea pigs were found to be connected by junctional complexes. No cell discontinuities or gaps were observed by scanning or transmission electron microscopy. These results are not in accordance with previous studies. They were achieved by in vivo vascular perfusion fixation, handling of cochleae in protective specimen carriers, thiocarbohydrazide treatment and continuous dehydration. Findings in the present study indicate that the interepithelial space between the epithelial and mesothelial cell layers constitutes a specific compartment which must be considered when examining solute transport over Reissner's membrane.
Insights
Mesothelial cells in guinea pig Reissner
Area of Science:
- Otolaryngology
- Cell Biology
- Membrane Physiology
Background:
- Previous studies suggested discontinuities in Reissner's membrane.
- Understanding Reissner's membrane structure is crucial for inner ear fluid balance.
Purpose of the Study:
- To investigate the ultrastructure of Reissner's membrane mesothelial cells.
- To clarify the presence or absence of gaps between Reissner's membrane cells.
- To define the interepithelial space's role in solute transport.
Main Methods:
- In vivo vascular perfusion fixation of guinea pig cochleae.
- Scanning and transmission electron microscopy.
- Specialized sample preparation including thiocarbohydrazide treatment and continuous dehydration.
Main Results:
- Mesothelial cells of Reissner's membrane are connected by junctional complexes.
- No cell discontinuities or gaps were observed.
- The interepithelial space is a distinct compartment.
Conclusions:
- Reissner's membrane exhibits a continuous cellular layer.
- The interepithelial space is a significant factor in solute transport across Reissner's membrane.
- Methodological improvements were key to these findings.