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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
Published on: March 7, 2018
Systematic analysis of posttranscriptional gene expression
Adam R Morris1, Neelanjan Mukherjee1, Jack D Keene1
1University Program in Genetics and Genomics, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA.
Wiley Interdisciplinary Reviews. Systems Biology and Medicine
|September 14, 2010
Summary
Understanding the ribonome, the dynamic network of RNA and proteins, reveals coordinated gene regulation. This reveals how RNA-binding proteins and ncRNAs control messenger RNA groups, impacting cellular functions and disease.
Area of Science:
- Molecular Biology
- Systems Biology
- Genetics
Background:
- The eukaryotic transcriptome results from complex transcriptional and posttranscriptional regulation.
- The ribonome, a dynamic network of ribonucleoproteins, involves interactions between RNA-binding proteins (RBPs), noncoding regulatory RNAs (ncRNAs), and messenger RNAs (mRNAs).
- Messenger RNAs can be organized into modular groups, termed posttranscriptional RNA operons or regulons (PTROs), which coordinate functionally related proteins.
Purpose of the Study:
- To examine methods for elucidating PTROs and their regulatory mechanisms.
- To investigate the dynamic systems governing PTROs and their collective response to cellular changes.
- To highlight the importance of ribonome studies for understanding cellular processes and disease.
Main Methods:
- Review of systems biology studies on gene expression.
- Analysis of RNA-binding protein and ncRNA interactions within the ribonome.
- Examination of mRNA stability and translation coordination within PTROs.
Main Results:
- PTROs coordinate mRNAs encoding functionally related proteins through RBPs and ncRNAs.
- mRNA components within PTROs bind collectively in dynamic 'states' to respond to cellular perturbations.
- Cellular mRNA abundance alone may not fully reflect the functional status of a biological system.
Conclusions:
- Understanding the ribonome offers greater insight into growth, differentiation, and disease than solely examining mRNA abundance.
- The coordinated regulation within PTROs is a key aspect of eukaryotic gene expression.
- Dynamic interactions within the ribonome are crucial for cellular response and function.
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