Gene silencing mediated by magnetic lipospheres tagged with small interfering RNA
Pablo del Pino1, Almudena Munoz-Javier, Dialechti Vlaskou
1Fachbereich Physik und Wissenschaftliches Zentrum für Materialwissenschaften, Philipps Universität Marburg, Renthof 7, 35037 Marburg, Germany.
Abstract:
Lipospheres made from soy bean oil and a combination of the cationic lipid Metafectene and the helper lipid dioleoylphosphatidyl-ethanolamine were functionalized with magnetic nanoparticles (NPs) and small interfering RNA (siRNA). The resulting magnetic lipospheres loaded with siRNA are proven here as efficient nonviral vectors for gene silencing. Embedding magnetic NPs in the shell of lipospheres allows for magnetic force-assisted transfection (magnetofection) as well as magnetic targeting in both static and fluidic conditions mimicking the bloodstream.
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