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Related Experiment Video

Updated: Jun 8, 2026

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

Functional gene discovery using RNA interference-based genomic screens to combat pathogen infection.

Elizabeth Hong-Geller¹, Sofiya N Micheva-Viteva

¹Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87544, USA. ehong@lanl.gov

Current Drug Discovery Technologies

|September 15, 2010

Summary

Antimicrobial resistance is a growing crisis. RNA interference (RNAi) genomic screens identify host genes manipulated by pathogens, offering new therapeutic targets to combat infectious diseases.

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Area of Science:

Genomics
Molecular Biology
Infectious Diseases

Background:

Antibiotic resistance is accelerating due to widespread use, creating a public health crisis.
Conventional antibiotic discovery methods are failing to keep pace with evolving drug-resistant pathogens.
Identifying host factors essential for infection offers a novel strategy for therapeutic development.

Purpose of the Study:

To review RNA interference (RNAi)-based genomic screening strategies.
To investigate host-pathogen interactions and identify novel therapeutic targets.
To explore host-based countermeasures against infectious diseases.

Main Methods:

Utilizing RNA interference (RNAi) for gene function analysis via mRNA destruction.
Developing high-throughput genome-wide RNAi screening platforms.
Employing human cell models to study infections by bacterial and viral pathogens.

Main Results:

RNAi screens have identified host genes manipulated by pathogens during infection.
These studies elucidate novel pathogen virulence mechanisms.
Functional genomics approaches reveal potential targets for host-directed therapies.

Conclusions:

RNAi-based genomic screens are powerful tools for understanding host-pathogen interactions.
Identifying essential host genes provides a basis for designing novel therapeutics.
Host-targeted inhibitors can block or mitigate the effects of pathogen infection, addressing the antimicrobial resistance crisis.