E, Z and positional-monoenoic phyto-fatty acids influencing membrane fluidity: DSC and NMR experiments

The membrane fluidity of biological tissues is highly influenced by the π-bond position and isomeric configuration in the long chain of phyto-fatty acids (FAs). Z, E and positional isomeric monoenoic lipids, i.e. the phytomolecules oleic (OA), elaidic (EA), vaccenic acid (TV) and its Z-isomer (CV), have been evaluated for their effects on the fluidity of cellular membranes. To this purpose the Differential Scanning Calorimetry (DSC) and Deuterium Nuclear Magnetic Resonance ((2)H-NMR), are suitable techniques to understand the supramolecular lamellar structure during the order (gel)-disorder (fluid) transition. It was found that the presence of CV concentration, induces the biomimetic system to reach the first step to fluid phase earlier than the membrane containing OA. DSC showed that the endothermic peak onset of the membrane containing CV occurs at a lower temperature than that of a membrane containing an equal amount of OA. (2)H-NMR investigation confirmed the last statement. In fact the study of the main phase transition of the two different systems, revealed that model membrane containing a 3% (w/w) of CV goes in ripple phase, i.e. the first step to the fluid state, at a lower temperature as compared to the membrane of an identical system with OA.