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Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Tuberculosis01:23

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Tuberculosis (TB) remains a significant global health concern, primarily targeting the lungs and spreading through airborne transmission. Infection begins when aerosolized droplet nuclei, expelled by an individual with active TB, are inhaled by another person. These microscopic particles carry Mycobacterium tuberculosis, the causative agent of TB. Upon reaching the alveoli, the bacilli are engulfed by alveolar macrophages. However, due to their specialized lipid-rich cell wall, these pathogens...
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Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
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Serum Laboratory Studies, Stool Test, Breath Test01:30

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Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
Pulmonary Tuberculosis I01:29

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Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
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Related Experiment Videos

Diagnostic markers for tuberculosis ascites: a preliminary study.

Rajpal S Kashyap1, Sonali M Saha, Khushboo J Nagdev

  • 1Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, Maharashtra, India.

Biomarker Insights
|September 15, 2010
PubMed
Summary

Diagnosing tuberculosis (TB) ascites is challenging. This study identified key protein markers, including heat shock proteins and the Ag 85 complex, in ascitic fluid for improved TB ascites diagnosis.

Keywords:
M. tuberculosis antigensTB ascitesheat shock proteins

Related Experiment Videos

Area of Science:

  • Proteomics
  • Infectious Diseases
  • Biochemistry

Background:

  • Tuberculosis (TB) ascites diagnosis is difficult, leading to delayed treatment and high mortality.
  • Identifying reliable biomarkers for TB ascites is crucial for timely intervention.

Purpose of the Study:

  • To identify specific protein markers in ascitic fluid for the diagnosis of TB ascites.
  • To improve the diagnostic accuracy and speed of TB ascites detection.

Main Methods:

  • Utilized a comprehensive quantitative proteomic screening system.
  • Employed Two-Dimensional Electrophoresis, liquid chromatography-mass spectrometry/mass spectrometry, immunoblot analysis, and ELISA.

Main Results:

  • Identified several protein antigens of interest, including the antigen 85 (Ag 85) complex, Mycobacterium tuberculosis (MTB) heat shock protein 65 (65-kDa HSP), MTB heat shock protein 14 (14-kDa HSP), and MTB heat shock protein 71 (71-kDa HSP).
  • ELISA confirmed higher detection rates of these antigens in TB ascites patients compared to non-TB ascites patients.

Conclusions:

  • The 65-kDa HSP, 71-kDa HSP, 14-kDa HSP, and Ag 85 complex proteins show potential as valuable diagnostic markers for TB ascites.
  • These protein markers could aid in the early and accurate diagnosis of TB ascites, potentially reducing mortality.