Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Low-dose heparin as an antithrombotic agent.

G F Gensini1, F Bonechi, A M Gori

  • 1Clinica Medica I, University of Florence, Italy.

Haemostasis
|January 1, 1990
PubMed
Summary

Low-dose heparin administration enhances the vessel wall's natural antithrombotic properties by binding to endothelial receptors. This effect improves thrombin and factor Xa inactivation, independent of circulating heparin levels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CDK9 inhibition as an effective therapy for small cell lung cancer.

Cell death & disease·2024
Same author

Clinical impact of high platelet reactivity in patients with atrial fibrillation and concomitant percutaneous coronary intervention on dual or triple antithrombotic therapy.

Journal of thrombosis and thrombolysis·2023
Same author

Prevalence and in-hospital outcome of patients with sepsis in an internal medicine ward.

La Clinica terapeutica·2021
Same author

Fever, abdominal pain, and lymphocytic ascites in a young immigrant from western Africa.

La Clinica terapeutica·2020
Same author

Mediterranean, but not lacto-ovo-vegetarian, diet positively influence circulating progenitor cells for cardiovascular prevention: The CARDIVEG study.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2019
Same author

Effects of a 3-month dietary intervention with a lacto-ovo-vegetarian diet on vitamin B<sub>12</sub> levels in a group of omnivores: results from the CARDIVEG (Cardiovascular Prevention with Vegetarian Diet) study.

The British journal of nutrition·2018

Area of Science:

  • Pharmacology
  • Vascular Biology
  • Thrombosis Research

Background:

  • Heparin is known to possess anticoagulant properties.
  • The interaction of heparin with endothelial receptors and its downstream effects on coagulation factors are not fully elucidated.
  • Subcutaneous administration of heparin is a common therapeutic route.

Purpose of the Study:

  • To investigate the binding of subcutaneously administered heparin to endothelial receptors in rabbits and humans.
  • To assess the impact of low-dose heparin on the inactivation of thrombin and factor Xa by the endothelium.
  • To evaluate the antithrombotic effects of heparin independent of its presence in circulating blood.

Main Methods:

  • Subcutaneous administration of N-sulphonate 35S-heparin in rabbits and standard heparin in humans.

Related Experiment Videos

  • Measurement of heparin binding to endothelial receptors.
  • Assessment of thrombin and factor Xa inactivation.
  • Quantification of plasma fibrinopeptide A levels and 125I-fibrinogen turnover.

    • Main Results:

    • Heparin binds to endothelial receptors in both rabbits and humans following subcutaneous administration.
    • Bound heparin in rabbits persisted for at least 24 hours, enhancing thrombin and factor Xa inactivation.
    • Two weeks of subcutaneous heparin in humans reduced fibrinopeptide A levels and normalized fibrinogen turnover.
    • Antithrombin-enhancing activity persisted longer than anti-Xa activity.

    Conclusions:

    • Low-dose heparin administration significantly enhances the antithrombotic properties of the vessel wall.
    • Heparin's pro-coagulant factor inactivation is mediated through endothelial binding.
    • Therapeutic benefits of heparin can be achieved through endothelial mechanisms, independent of circulating drug levels.