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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jun 8, 2026

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
13:36

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

MicroRNA-21 protects neurons from ischemic death.

Ben Buller1, Xianshuang Liu, Xinli Wang

  • 1Department of Neurology, Henry Ford Hospital, Detroit, MI, USA.

The FEBS Journal
|September 16, 2010
PubMed
Summary

MicroRNA-21 (miR-21) protects against brain damage after stroke. Upregulated miR-21 in ischemic neurons reduces cell death by targeting FASLG, suggesting miR-21 as a potential stroke therapy.

Related Experiment Videos

Last Updated: Jun 8, 2026

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
13:36

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression.
  • The role of miRNAs, specifically microRNA-21 (miR-21), in cerebral ischemia remains largely unknown.
  • miR-21 is recognized as an anti-apoptotic factor in various biological contexts.

Purpose of the Study:

  • To investigate the expression and function of miR-21 in the context of stroke in a rat model.
  • To determine if miR-21 plays a neuroprotective role following ischemic injury.
  • To elucidate the molecular mechanism underlying miR-21's effect, focusing on its interaction with FASLG.

Main Methods:

  • In situ hybridization and laser capture microdissection coupled with real-time RT-PCR were used to quantify miR-21 expression in ischemic rat brains.
  • In vitro studies involved manipulating miR-21 levels in cultured cortical neurons subjected to oxygen-glucose deprivation.
  • A luciferase reporter assay was employed to validate the interaction between miR-21 and the 3'-UTR of Faslg mRNA.

Main Results:

  • miR-21 expression was significantly upregulated in neurons within the ischemic boundary zone of the rat brain 2 and 7 days post-stroke.
  • Overexpression of miR-21 in cultured neurons reduced apoptosis induced by oxygen-glucose deprivation.
  • Inhibition of endogenous miR-21 exacerbated cell death, while miR-21 overexpression decreased FASLG levels, confirmed by reporter assays targeting Faslg mRNA.
  • The neuroprotective effect of miR-21 was linked to the downregulation of FASLG, a pro-apoptotic ligand.

Conclusions:

  • miR-21 is upregulated in neurons after stroke and exerts significant neuroprotection against ischemic cell death.
  • The anti-apoptotic function of miR-21 in stroke is mediated, at least in part, by the downregulation of FASLG.
  • These findings highlight miR-21 as a promising therapeutic target for stroke treatment.