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Published on: June 6, 2025
MicroRNA-146a expresses in interleukin-17 producing T cells in rheumatoid arthritis patients
Takuya Niimoto1, Tomoyuki Nakasa, Masakazu Ishikawa
1Department of Orthopaedic Surgery, Programs for Applied Biomedicine, Division of Clinical Medical Science, Graduate School of Biomedical Sciences, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551 Japan. tnakasa@smn.enjoy.ne.jp
Background:
Interleukin (IL)-17 is an important factor in rheumatoid arthritis (RA) pathogenesis. MicroRNA (miRNA)s are a family of non coding RNAs and associated with human diseases including RA. The purpose of this study is to identify the miRNAs in the differentiation of IL-17 producing cells, and analyze their expression pattern in the peripheral blood mononuclear cells (PBMC) and synovium from RA patients.
Methods:
IL-17 producing cells were expanded from CD4+T cell. MiRNA microarray was performed to identify the miRNAs in the differentiation of IL-17 producing cells. Quantitative polymerase chain reaction was performed to examine the expression patterns of the identified miRNAs in the PBMC and synovium from RA and osteoarthritis (OA) patients. Double staining combining in situ hybridization and immunohistochemistry of IL-17 was performed to analyze the expression pattern of identified miRNA in the synovium.
Results:
Six miRNAs, let-7a, miR-26, miR-146a/b, miR-150, and miR-155 were significantly up regulated in the IL-17 producing T cells. The expression of miR-146a and IL-17 was higher than in PBMC in the patients with low score of Larsen grade and short disease duration. MiR-146a intensely expressed in RA synovium in comparison to OA. MiR-146a expressed intensely in the synovium with hyperplasia and high expression of IL-17 from the patients with high disease activity. Double staining revealed that miR-146a expressed in IL-17 expressing cells.
Conclusion:
These results indicated that miR-146a was associated with IL-17 expression in the PBMC and synovium in RA patients. There is the possibility that miR-146a participates in the IL-17 expression.
Insights
MicroRNA-146a is linked to Interleukin-17 in rheumatoid arthritis (RA) patients. This microRNA may play a role in regulating IL-17 expression within immune cells and joint tissues.
Area of Science:
- Immunology
- Molecular Biology
- Rheumatology
Background:
- Interleukin-17 (IL-17) is a key factor in rheumatoid arthritis (RA) pathogenesis.
- MicroRNAs (miRNAs) are implicated in various human diseases, including RA.
Purpose of the Study:
- Identify miRNAs involved in IL-17-producing cell differentiation.
- Analyze miRNA expression patterns in peripheral blood mononuclear cells (PBMCs) and synovium from RA patients.
Main Methods:
- Expansion of IL-17-producing CD4+ T cells.
- miRNA microarray analysis to identify key miRNAs.
- Quantitative PCR and double staining (in situ hybridization/immunohistochemistry) to assess miRNA and IL-17 expression in patient samples.
Main Results:
- Six miRNAs (let-7a, miR-26, miR-146a/b, miR-150, miR-155) were upregulated in IL-17-producing T cells.
- miR-146a and IL-17 expression was higher in PBMCs of RA patients with lower disease severity.
- miR-146a showed intense expression in RA synovium compared to osteoarthritis (OA), particularly in inflamed tissues with high IL-17 levels.
Conclusions:
- miR-146a is associated with IL-17 expression in both PBMCs and synovium of RA patients.
- miR-146a may participate in the regulation of IL-17 expression in RA.
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