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Updated: Jun 8, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
Published on: March 17, 2016
Age-dependent prevalence of equid herpesvirus 5 infection
Maria Luisa Marenzoni1, Giacomo Coppola, Margherita Maranesi
1Sports Horse Research Centre, Department of Experimental Science and Applied Biotechnology, Faculty of Veterinary Medicine, University of Perugia, via S. Costanzo 4, 06126, Perugia, Italy. marialuisa.marenzoni@unipg.it
Abstract:
Equid herpesvirus 5 (EHV-5) infection was detected in a farm in Italy by the use of a semi-nested polymerase chain reaction (PCR) targeting glycoprotein B of EHV-5 on nasal swabs and blood samples of clinically healthy and randomly selected Lipizzaner horses (n = 55). Twenty-five horses at the age of 4-17 years and 30 at an age of 1-3 years were sampled once. The association of the infection with these age-groups and the gender of the horses was investigated. The apparent prevalence of EHV-5 infection was significantly different between age-cohorts: it was higher in the younger group of horses with 73,3% and 80% positives in nasal swabs and blood respectively, compared to 40% of nasal swabs and 20% of blood in the older horses. An age-dependence therefore was observed: the young age is more frequently associated with EHV-5 infection.
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