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Updated: Jun 8, 2026
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Measuring Single-Cell Aging with an Imaging-based Biomarker of Chromatin and Epigenetic Aging
Published on: January 30, 2026
Plasma biomarkers of mouse aging
1Edison Biotechnology Institute, Ohio University, 1 Water Tower Drive, The Ridges, Athens, OH 45701, USA.
Age (Dordrecht, Netherlands)
|September 16, 2010
Summary
Researchers identified novel aging biomarkers by analyzing plasma proteins in male mice. These protein changes, including transthyretin and albumin isoforms, could help detect premature aging and improve healthspan.
Area of Science:
- Gerontology
- Proteomics
- Biomarker Discovery
Background:
- Normal aging involves physiological changes like reduced immunity and increased disease susceptibility.
- Identifying reliable biomarkers is crucial for understanding and intervening in the aging process.
Purpose of the Study:
- To discover novel plasma protein biomarkers associated with normal aging in mice.
- To analyze longitudinal changes in protein isoforms during aging.
Main Methods:
- Longitudinal analysis of plasma proteins from male mice (2-19 months old).
- Two-dimensional gel electrophoresis coupled with mass spectrometry (MS, MS/MS, LC-MS/MS) for protein identification.
- Quantification and statistical analysis of protein isoform expression levels.
Main Results:
- Thirty-nine protein spots, representing six distinct proteins, were identified.
- Thirteen protein isoforms showed significant age-related changes.
- Examples include increased transthyretin, haptoglobin, and immunoglobulin kappa chain isoforms, and decreased peroxiredoxin-2, serum amyloid protein A-1, and albumin isoforms.
Conclusions:
- Identified protein isoforms serve as potential biomarkers for normal aging in mice.
- These biomarkers may offer avenues for therapeutic interventions targeting premature aging and enhancing healthspan.
- Further validation in human studies is warranted.
