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Lack of p16 gene mutations in gastric cancers in Kashmir

Sheikh Alina Bashir1, Arshad Ahmad Pandith, Adfar Yousuf

  • 1Department of Biotechnology Jamia Hamdard, Hamdard Nagar New Delhi, India.

Asian Pacific Journal of Cancer Prevention : APJCP
|September 17, 2010
PubMed
Abstract

Insights

Gastric carcinoma is common in Kashmir, but this study found no p16 gene mutations or deletions in 84 patients. The p16 gene does not appear to be a factor in these gastric cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric carcinoma presents a high incidence in the Kashmiri population.
  • The p16 gene is a tumor suppressor gene frequently altered in various cancers.
  • Investigating genetic alterations in gastric cancer is crucial for understanding its pathogenesis.

Purpose of the Study:

  • To determine the frequency of p16 gene mutations and homozygous deletions in gastric carcinomas.
  • To assess the role of the p16 gene in gastric cancer development within the Kashmiri population.

Main Methods:

  • Screening of 84 gastric carcinoma patients using Single Strand Conformation Polymorphism (SSCP) and DNA sequencing.
  • Utilizing Polymerase Chain Reaction (PCR) to detect homozygous deletions of the p16 gene.

Main Results:

  • No mutations in the p16 gene were detected in any of the 84 gastric carcinoma cases.
  • While mobility shifts were observed in SSCP for two samples, DNA sequencing confirmed no mutations.
  • PCR analysis did not reveal any homozygous deletions of the p16 gene in the studied cases.

Conclusions:

  • Despite high gastric carcinoma rates in Kashmir, alterations in the p16 gene (mutations or deletions) do not seem to be involved.
  • The p16 gene is unlikely to be a significant contributor to gastric carcinogenesis in this specific population.

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