Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR - Polymerase Chain Reaction

PCR - Polymerase Chain Reaction

PCR

PCR

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Understanding the qPCR Standard Curve: From Assay Validation to Absolute Quantification and Variance PCR.

International journal of molecular sciences·2026

Same author

Quantification Revisited: What qPCR Efficiency Models Reveal About Data Analysis Integrity.

International journal of molecular sciences·2026

Same author

Real-Time Reverse Transcription Quantitative PCR (RT-qPCR) Methodological Standards and Reporting Practices.

Clinical chemistry·2026

Same author

MISEV and MIQE: integrating domain-specific and general standards to strengthen extracellular vesicle biomarker research.

Extracellular vesicles and circulating nucleic acids·2026

Same author

When Two-Fold Is Not Enough: Quantifying Uncertainty in Low-Copy qPCR.

International journal of molecular sciences·2025

Same author

Discovery, phylogenetic, and comparative genomic analysis of novel avian gammacoronaviruses identified in feral pigeons (<i>Columba livia domestica</i>).

Journal of virology·2025

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

Developments in real-time PCR research and molecular diagnostics.

Stephen A Bustin¹

¹Blizard Institute for Cell and Molecular Science, Bart's and The London School of Medicine and Dentistry, University of London, London, UK and 3rd Floor Alexandra Wing, Royal London Hospital, London, UK. s.a.bustin@qmul.ac.uk

Expert Review of Molecular Diagnostics

|September 17, 2010

Summary

Quantitative real-time PCR (qPCR) is popular in life sciences and diagnostics due to new methods and applications. This meeting showcased current trends, novel approaches, and critical considerations for qPCR assay design and quality control.

More Related Videos

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Area of Science:

Life Science Research
Clinical Diagnostics

Background:

Quantitative real-time PCR (qPCR) technology is widely adopted across diverse research and diagnostic fields.
Its popularity stems from advancements in methods, instrumentation, and applications.

Purpose of the Study:

To showcase emerging methodologies and applications in qPCR.
To provide an overview of current trends and innovative strategies in the field.
To address key challenges in qPCR assay development, including optimization and quality control.

Main Methods:

The content is derived from a meeting highlighting new developments in qPCR.
Focus on methods, instruments, and applications.
Discussion of assay design, optimization, and quality control.

Main Results:

A snapshot of current trends and novel approaches in qPCR was presented.
Important issues regarding assay design, optimization, and quality control were discussed.

Conclusions:

Quantitative real-time PCR is a versatile technology with ongoing innovation.
Effective assay design and rigorous quality control are crucial for reliable qPCR results in research and diagnostics.