Monitoring platelet dependent thrombin generation in mice
Yesim Dargaud1, Henri M H Spronk, Peter Leenders
1Laboratory for Clinical Thrombosis and Haemostasis, Department of Internal Medicine, and Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands. ydargaud@univ-lyon1.fr
Abstract:
Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2μM inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo- and hypercoagulability.
