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Published on: October 24, 2016
Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097
Bruno Guedes Fonseca1, Rondinele de Oliveira Moutta, Flavio de Oliveira Ferraz
1Deptamento de Biotecnologia Lorena, Universidade de São Paulo/Escola de Engenharia de Lorena, Lorena, São Paulo, 12.602-810, Brazil.
Abstract:
This work had as its main objective to contribute to the development of a biological detoxification of hemicellulose hydrolysates obtained from different biomass plants using Issatchenkia occidentalis CCTCC M 206097 yeast. Tests with hemicellulosic hydrolysate of sugarcane bagasse in different concentrations were carried out to evaluate the influence of the hydrolysate concentration on the inhibitory compounds removal from the sugarcane bagasse hydrolysate, without reduction of sugar concentration. The highest reduction values of inhibitors concentration and less sugar losses were observed when the fivefold concentrated hydrolysate was treated by the evaluated yeast. In these experiments it was found that the high sugar concentrations favored lower sugar consumption by the yeast. The highest concentration reduction of syringaldehyde (66.67%), ferulic acid (73.33%), furfural (62%), and 5-HMF (85%) was observed when the concentrated hydrolysate was detoxified by using this yeast strain after 24 h of experimentation. The results obtained in this work showed the potential of the yeast Issatchenkia occidentalis CCTCC M 206097 as detoxification agent of hemicellulosic hydrolysate of different biomass plants.
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