Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

PCR

PCR

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR - Polymerase Chain Reaction

PCR - Polymerase Chain Reaction

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prokaryotically expressed IBDV VP2 virus-like particles: Stability and protective efficacy.

Microbial pathogenesis·2026

Same author

A ferritin-based mosaic-like nanovaccine elicits effective cross-protection against H1N1 and H3N2 swine influenza viruses.

Journal of nanobiotechnology·2026

Same author

A Novel Pathogen of an Emerging Infectious Disease (Large Kidney Disease) in Farmed Blue Foxes.

Transboundary and emerging diseases·2025

Same author

Development of a quantum dots based immunochromatographic strip for rapid and on-site detection of African swine fever virus.

Microbial pathogenesis·2024

Same author

Development of a double antibody sandwich ELISA method for the quantitative detection of serum C-reactive protein based on nanobody.

Microbial pathogenesis·2024

Same author

Dual-RPA assay for rapid detection and differentiation of E.granulosus and E.multilocularis.

Microbial pathogenesis·2024

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

Applications of loop-mediated isothermal DNA amplification.

Shijun Fu¹, Guanggang Qu, Shijin Guo

¹Key Laboratory of Preventive Veterinary Medicine and Animal Biotechnology, Binzhou Animal Husbandry and Veterinary Research Academy, Binzhou 256600, People's Republic of China. fsj729@yahoo.com.cn

Applied Biochemistry and Biotechnology

|September 17, 2010

Summary

Loop-mediated isothermal amplification (LAMP) offers a simple, rapid, and specific method for nucleic acid analysis. Its applications span pathogen detection, GMOs, cancer diagnostics, and sex identification, requiring no expensive equipment.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Related Experiment Videos

Last Updated: Jun 8, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Area of Science:

Molecular Biology
Biotechnology
Diagnostics

Background:

Loop-mediated isothermal amplification (LAMP) has emerged as a significant nucleic acid analysis technique over the past decade.
LAMP is recognized for its simplicity, speed, high efficiency, and specificity.
It offers advantages over conventional PCR and real-time PCR, including reduced equipment needs and fewer preparation steps.

Purpose of the Study:

To summarize the diverse applications of the LAMP method.
To highlight the advantages of LAMP in various analytical fields.
To provide an overview of LAMP's utility in pathogen detection, GMO analysis, tumor diagnostics, and sex identification.

Main Methods:

The LAMP method utilizes a DNA polymerase and four unique primers targeting six distinct sequences on the DNA.
It operates under isothermal conditions, eliminating the need for thermal cycling equipment.
The assay is designed for ease of use and rapid results.

Main Results:

LAMP has demonstrated broad applicability across multiple domains.
Successful applications include the detection of pathogenic microorganisms.
Its utility extends to identifying genetically modified ingredients, detecting tumors, and determining embryo sex.

Conclusions:

The LAMP method is a versatile and powerful tool for nucleic acid amplification and analysis.
Its ease of use, efficiency, and specificity make it suitable for a wide range of diagnostic and research purposes.
LAMP represents a valuable advancement in molecular diagnostics, offering practical solutions for various applications.