Related Experiment Video

Updated: Jun 8, 2026

Reductive Electropolymerization of a Vinyl-containing Poly-pyridyl Complex on Glassy Carbon and Fluorine-doped Tin Oxide Electrodes
09:17

Reductive Electropolymerization of a Vinyl-containing Poly-pyridyl Complex on Glassy Carbon and Fluorine-doped Tin Oxide Electrodes

Published on: January 30, 2015

Electroanalysis of trimethoprim on metalloporphyrin incorporated glassy carbon electrode

Leena Rajith1, Krishnapillai Girish Kumar

  • 1Department of Applied Chemistry, Cochin University of Science and Technology, Kochi, Kerala, India.

Drug Testing and Analysis
|September 17, 2010
PubMed

Insights

A novel electrochemical sensor using a metalloporphyrin-modified electrode enables sensitive and selective detection of Trimethoprim (TMP). This method offers a low detection limit and stability for analyzing TMP in pharmaceutical and biological samples.

Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Trimethoprim (TMP) is a vital antibiotic for urinary tract infections, but its idiosyncratic reactions necessitate reliable detection methods.
  • Existing methods for TMP determination may lack sensitivity or selectivity.
  • Development of advanced electrochemical sensors is crucial for therapeutic drug monitoring and quality control.

Purpose of the Study:

  • To develop and characterize a novel electrochemical sensor for the sensitive and selective determination of Trimethoprim (TMP).
  • To investigate the electrocatalytic activity of a metalloporphyrin-modified glassy carbon electrode for TMP oxidation.
  • To evaluate the sensor's performance, including detection limit, linearity, repeatability, stability, and interference.

Main Methods:

  • Fabrication of a glassy carbon electrode modified with [5,10,15,20-tetrakis(4-methoxyphenyl)porphyrinato]Mn(III)chloride (TMOPPMn(III)Cl).
  • Electrochemical analysis of TMP using differential pulse voltammetry (DPV) in phosphate buffer solution.
  • Optimization and characterization of electrochemical parameters, including pH dependence and adsorption control.
  • Assessment of sensor performance metrics: linearity, detection limit, repeatability, operational stability, and interference studies.

Main Results:

  • The TMOPPMn(III)Cl modified electrode exhibited enhanced electrocatalytic activity for TMP oxidation compared to the bare electrode.
  • The electro-oxidation of TMP was found to be irreversible, pH-dependent, and adsorption-controlled.
  • A wide linear range (6 × 10⁻⁸ - 1 × 10⁻⁶ M) and a very low detection limit (3 × 10⁻⁹ M) were achieved.
  • The sensor demonstrated good repeatability (RSD = 3.2%), operational stability (20 days), and selectivity, with no interference from sulfamethoxazole.

Conclusions:

  • The developed TMOPPMn(III)Cl/GCE sensor provides a highly sensitive and selective platform for electrochemical determination of TMP.
  • The sensor's robustness and ability to function in complex matrices like urine and tablets highlight its practical applicability.
  • This electrochemical approach offers a promising alternative for routine analysis and quality control of Trimethoprim.

More Related Videos

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications10:48

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

Published on: July 28, 2021

Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes08:32

Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes

Published on: June 30, 2019

Related Concept Videos

Voltammetry: Stripping Methods01:13

Voltammetry: Stripping Methods

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
Electrodeposition01:08

Electrodeposition

Electrodeposition is a technique used to separate an analyte from interferents by electrochemical processes. Here, the analyte is a metal ion that can be deposited on an electrode immersed in the sample solution. The electrochemical setup consists of an anode and a cathode. When an electric current is applied to the setup, oxidation occurs at the anode. At the cathode, which consists of a large metal surface, metal ions undergo reduction and deposit onto the surface.
Electrodeposition can...
Electrogravimetric Analysis: Overview01:30

Electrogravimetric Analysis: Overview

Electrogravimetric analysis measures the weight of an analyte deposited electrolytically onto a suitable working electrode. This method involves applying a potential to a pre-weighed electrode submerged in a solution, which results in the desired substance being deposited through reduction at the cathode or oxidation at the anode. The electrode's weight is recorded after deposition, and the difference in weight gives the analyte's weight in the solution.
To test the completeness of the...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...