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Related Experiment Video

Updated: Jun 8, 2026

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
12:59

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

Published on: February 15, 2020

Oxidative stress in cataractogenesis.

Sonja Cekić1, Gordana Zlatanović, Tatjana Cvetković

  • 1Eye Clinic, Clinic Centre Niš, Bulevar dr Zorana Đinđića 48, 18000 Niš, Serbia.

Bosnian Journal of Basic Medical Sciences
|September 18, 2010
PubMed
Summary

Cataract patients show altered levels of antioxidants and lipid peroxidation products in their serum compared to controls. These changes in total sulfhydryl groups and malondialdehyde (MDA) indicate oxidative stress associated with age-related cataracts.

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Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
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Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Published on: November 28, 2012

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Last Updated: Jun 8, 2026

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
12:59

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

Published on: February 15, 2020

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
13:56

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Published on: November 28, 2012

Area of Science:

  • Ophthalmology
  • Biochemistry
  • Oxidative Stress Research

Background:

  • Cataract formation is linked to oxidative stress.
  • Antioxidants like total sulfhydryl groups (SH) and glutathione (GSH) play a protective role.
  • Malondialdehyde (MDA) is a marker of lipid peroxidation and oxidative damage.

Purpose of the Study:

  • To compare serum and lens levels of total SH groups, GSH, and MDA in patients with age-related cataracts versus controls.
  • To investigate potential differences in these markers among various types of age-related cataracts.

Main Methods:

  • Serum and lens nucleocortical parts were analyzed from 38 cataract patients and 38 controls.
  • Total SH groups measured using Ellman's method.
  • GSH and MDA (TBARS) quantified using Sedlak and Lindsay's method and thiobarbituric acid assay, respectively.
  • Statistical analysis performed using Student's t-test (p<0.05).

Main Results:

  • Significantly lower total SH groups (p<0.05) and higher MDA levels (p<0.001) were observed in the serum of cataract patients compared to controls.
  • No significant differences in total SH groups or MDA were found in serum or lens between different types of age-related cataracts.
  • No significant differences in GSH levels were found in the lens between groups.

Conclusions:

  • Serum levels of total SH groups and MDA are significantly altered in patients with age-related cataracts, suggesting a role for oxidative stress.
  • Oxidative stress markers in serum do not significantly differ between various types of age-related cataracts.
  • Further research is needed to explore the role of GSH in the lens concerning cataract development.