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Updated: Jun 8, 2026
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
A novel technique to center the donor punch for descemet stripping endothelial keratoplasty
Marshall Bowes Hamill1, Kevin Shuo Zhang
1Baylor College of Medicine, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA. mhamill@bcm.tmc.edu
Purpose:
This report describes a method to improve the accuracy of centration of the donor trephination for Descemet stripping endothelial keratoplasty.
Methods:
Using trypan blue, the donor corneal cap is outlined. The donor punch is centered on the actual cap rim rather than on a centration mark made either by the surgeon or by the eye bank preparing the precut tissue.
Results:
Using the described technique, eccentric donor trephination was reduced from 7.7% to 2.4%.
Conclusions:
We have described a method to improve the centration of donor trephination and reduce the incidence of eccentric donor punching.
