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Related Concept Videos

RNA Editing02:23

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Updated: Jun 8, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Site-specific photochemical RNA editing.

Kenzo Fujimoto1, Kaoru Konishi-Hiratsuka, Takashi Sakamoto

  • 1School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahi-dai, Nomi, Ishikawa, 923-1292, Japan. kenzo@jaist.ac.jp

Chemical Communications (Cambridge, England)
|September 18, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed photo-induced artificial RNA editing using special molecules. This non-enzymatic method precisely edits RNA, aiding the study of RNA functions and new drug development.

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09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • RNA editing is crucial for gene expression regulation.
  • Current RNA editing methods often rely on enzymes or lack specificity.
  • Understanding RNA functions, especially non-coding RNAs, requires precise manipulation tools.

Purpose of the Study:

  • To develop a novel, non-enzymatic method for artificial RNA editing.
  • To demonstrate the efficacy of photo-induced RNA editing using photo-reactive oligonucleotides.
  • To establish a sequence-specific RNA editing tool for functional and therapeutic applications.

Main Methods:

  • Synthesis of photo-reactive oligonucleotides incorporating 3-cyanovinylcarbazole nucleoside.
  • Application of photo-irradiation to induce site-specific RNA modification.
  • Characterization of the editing efficiency and specificity through molecular assays.

Main Results:

  • Successful demonstration of photo-induced artificial RNA editing.
  • Achieved sequence-specific RNA modification without enzymatic intervention.
  • Validated the use of 3-cyanovinylcarbazole nucleoside for photo-reactive oligonucleotide design.

Conclusions:

  • Photo-induced artificial RNA editing offers a powerful non-enzymatic approach.
  • This methodology significantly advances the study of RNA functions, including non-coding RNAs.
  • The developed technique holds promise for creating novel drugs based on sequence-specific RNA editing.