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Related Experiment Video

Updated: Jun 8, 2026

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
08:18

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Published on: December 31, 2014

c-Myc stimulates cell invasion by inhibiting FBX8 function.

Hyun Jung Cho1, Yun Jeong Oh, Junhye Kwon

  • 1Department of Biological Sciences, Sungkyunkwan University, Suwon, 440-746, Korea.

Molecules and Cells
|September 18, 2010
PubMed
Summary

The oncoprotein c-Myc binds to FBX8, a protein regulating cell invasion. c-Myc disrupts FBX8

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Last Updated: Jun 8, 2026

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
08:18

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Published on: December 31, 2014

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • c-Myc is a key oncoprotein regulating cell growth and division.
  • The precise mechanisms by which c-Myc drives tumorigenesis remain incompletely understood.
  • Understanding c-Myc's oncogenic pathways is crucial for developing targeted cancer therapies.

Purpose of the Study:

  • To identify novel proteins interacting with c-Myc.
  • To elucidate the role of these interactions in c-Myc-mediated tumorigenesis.
  • To investigate the functional consequences of the c-Myc/FBX8 interaction on cell invasion.

Main Methods:

  • Large-scale tandem repeat affinity purification was employed to identify c-Myc interacting proteins.
  • Co-immunoprecipitation and Western blotting confirmed the interaction between c-Myc and FBX8.
  • Cellular translocation assays and functional assays assessed the impact of c-Myc overexpression on FBX8 and ARF6.

Main Results:

  • F-box only protein 8 (FBX8) was identified as a novel binding partner of c-Myc.
  • The interaction between c-Myc and FBX8 is mediated by the c-Myc box II (MBII) region.
  • c-Myc overexpression altered FBX8 cellular localization and relieved FBX8-mediated inhibition of ADP-ribosylation factor 6 (ARF6) during cell invasion.

Conclusions:

  • FBX8 is a novel c-Myc binding protein.
  • c-Myc promotes cell invasion by disrupting the regulatory function of FBX8 on ARF6.
  • This finding provides new insights into the molecular mechanisms of c-Myc-driven oncogenesis and cell motility.