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Ribonuclease activities associated with purified foot and mouth disease virus
Archives of Virology
|January 1, 1978
Abstract:
Ribonuclease activities internally and externally associated with purified foot-and mouth disease virus were detected. The outer activity was easily removed by cesium chloride or by detergent (Sarkosyl). The inner activity is not removable by any procedure used and could be the enzyme responsible for the heterogeneity normally observed in the extracted FMDV-RNA. It is not known at present if both activities are related to the same or to different enzymes.
Insights
Foot-and-mouth disease virus (FMDV) contains two types of ribonuclease activities. An outer activity is removable, while an inner activity persists, potentially explaining FMDV-RNA heterogeneity.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Background:
- Foot-and-mouth disease virus (FMDV) is a significant pathogen affecting livestock.
- Understanding viral RNA processing is crucial for developing antiviral strategies.
Purpose of the Study:
- To investigate the ribonuclease (RNase) activities associated with purified FMDV.
- To characterize the location and properties of these RNase activities.
Main Methods:
- Purification of FMDV.
- Enzymatic assays to detect ribonuclease activity.
- Treatment with cesium chloride and Sarkosyl detergent to assess activity removal.
Main Results:
- Two distinct ribonuclease activities were detected: one external and one internal to the virus.
- The external activity was removed by cesium chloride or Sarkosyl.
- The internal activity remained associated with the virus after all tested treatments.
Conclusions:
- The internal ribonuclease activity is resistant to removal and may be responsible for the observed heterogeneity in extracted FMDV-RNA.
- Further research is needed to determine if the internal and external activities originate from the same or different enzymes.