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Updated: Jun 8, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
[Study on the epidemiological of rotavirus diarrhea in Lulong in 2008-2009]
Dan-Di Li1, Qiu-Li Yu, Shun-Xiang Qi
1State Key Laboratory of Molecular Virology and Genetic Engineering, Beijing 100052, China.
Objective:
To analyze the feature of epidemiological of rotavirus diarrhea in Lulong county, Hebei province.
Methods:
426 stool specimens were collected from inpatant with acute diarrhea from children less than 5 years old. Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR.
Results:
Rotavirus was detected in 202 of 426 (47.42%) specimens. Genotyping of rotavirus showed that G3 was predominant (57.9%), followed by Gmix (16.3%), G9 (14.9% ), G1 (7.9%), G4 (1%), G2 (0.5%), P-genotyping showed that P [8], Pmix, P [4], P [9], type were found in 58.4%, 28.7%, 6.9% and 1% respectively. The most common G/P combination identified was G3P [8].
Conclusion:
Group A rotaviruses was a major pathogen of diarrhea in Children in Lulong. G3P [8] was the predominant type in 2009, Gmix and Pmix abound, and G9 serotypes has become the second predominant after G3 strain in the region.
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