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Updated: Jun 8, 2026
Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
[Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin I]
Hong-Hui Shen1, Jun Hou, Yan Hu
1College of Life sciences, Jilin University, Changchun 130012, China.
Objective:
To develop a vector inserted with complete genome of poliovirus strain Sabin I.
Methods:
The 3 fragments of the complete genome of Sabin I was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified.
Results:
The complete genome of poliovirus strain Sabin I was successfully cloned into vector pWSK29 with 9 nucleotide mutations.
Conclusion:
The complete genome plasmid was constructed and it provided a basis for further research of the function of Sabin I.
