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Updated: Jun 8, 2026

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Gelatin-based laser direct-write technique for the precise spatial patterning of cells.

Nathan R Schiele¹, Douglas B Chrisey, David T Corr

¹Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York, USA.

Tissue Engineering. Part C, Methods

|September 21, 2010

Summary

This study introduces gelatin as a novel material for laser cell printing, enhancing cell viability and pattern precision. Gelatin coatings enable temporary support during printing and unobstructed growth post-transfer, overcoming limitations of traditional methods.

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Last Updated: Jun 8, 2026

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Area of Science:

Biotechnology
Cell Biology
Materials Science

Background:

Laser direct-writing is a key technique for patterning living cells in vitro.
Current materials like Matrigel™ have limitations for long-term cell culture applications.
Developing improved biomaterials is crucial for advancing cell-based research and tissue engineering.

Purpose of the Study:

To develop and evaluate a new laser cell printing approach using gelatin coatings.
To overcome the limitations associated with conventional materials in laser direct-writing.
To assess the efficacy of gelatin in supporting cell viability and precise patterning.

Main Methods:

Utilized gelatin coatings on the print ribbon and growth surface for laser direct-writing.
Printed human dermal fibroblasts using the gelatin-based approach.
Assessed cell viability, DNA integrity, and pattern fidelity post-transfer using microscopy and DNA damage assays.

Main Results:

Achieved high post-transfer viability (91% ± 3%) for human dermal fibroblasts.
Observed no double-strand DNA damage in printed cells.
Demonstrated that gelatin temporarily supports cell transfer and is removed upon incubation, allowing unobstructed cellular growth.
Confirmed precise cell placement, with cells maintaining registry within 5.6 ± 2.5 μm after gelatin removal.

Conclusions:

Gelatin is an effective and versatile biomaterial for laser direct-writing of living cells.
This gelatin-based method enhances cell viability and spatial patterning accuracy.
The approach holds significant potential for applications in tissue engineering, stem cell research, and cancer research.