Related Experiment Video

Updated: Jun 8, 2026

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
09:10

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

Ubiquitin-like protein MNSFβ/endophilin II complex regulates Dectin-1-mediated phagocytosis and inflammatory

Morihiko Nakamura1, Natsuko Watanabe

  • 1Department of Cooperative Medical Research, Collaboration Center, Shimane University, Izumo 693-8501, Japan. nkmr0515@med.shimane-u.ac.jp

Biochemical and Biophysical Research Communications
|September 21, 2010
PubMed

Insights

Monoclonal nonspecific suppressor factor β (MNSFβ) and endophilin II regulate macrophage phagocytosis via Dectin-1 signaling. Silencing these proteins enhances zymosan-induced TNFα production and IκBα degradation.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Post-translational modification by MNSFβ regulates cellular events.
  • MNSFβ conjugates to Bcl-G, impacting apoptosis.
  • MNSFβ also conjugates to endophilin II, inhibiting macrophage phagocytosis.

Purpose of the Study:

  • To investigate the mechanism of MNSFβ/endophilin II complex in zymosan phagocytosis.
  • To elucidate the role of MNSFβ/endophilin II in Dectin-1 signaling and TNFα production.

Main Methods:

  • Utilized Raw264.7 macrophage cell line.
  • Employed siRNA to silence endophilin II and MNSFβ.
  • Analyzed TNFα production and IκBα degradation in response to zymosan and Pam3CSK4.
  • Used anti-Dectin-1 mAb to assess Dectin-1 signaling involvement.

Main Results:

  • MNSFβ/endophilin II inhibition of phagocytosis is mediated by Dectin-1 signaling.
  • Silencing MNSFβ or endophilin II enhanced zymosan-induced TNFα production.
  • Silencing MNSFβ or endophilin II enhanced zymosan-induced IκBα degradation.
  • MNSFβ/endophilin II complex acts upstream of IKK activation, independent of TLR2 signaling.

Conclusions:

  • MNSFβ/endophilin II complex is a key regulator of Dectin-1-mediated phagocytosis and inflammatory responses.
  • The MNSFβ/endophilin II complex inhibits signaling pathways upstream of IKK activation.
  • This complex plays a crucial role in modulating macrophage responses to fungal components like zymosan.

More Related Videos

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization09:22

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Published on: November 27, 2014

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Related Concept Videos

Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized by phagocytes.
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...