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qPCR assays to quantify genes and gene expression associated with microbial perchlorate reduction

Susan K De Long1, Kerry A Kinney, Mary Jo Kirisits

  • 1The University of Texas at Austin, Department of Civil, Architectural, and Environmental Engineering, 1 University Station C1786, Austin, TX 78712, United States. Susan.De_Long@colostate.edu

Journal of Microbiological Methods
|September 21, 2010
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...