Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nondisjunction01:21

Nondisjunction

Nondisjunction is the failure of homologous chromosomes or sister chromatids to separate correctly and move to the opposite poles of the cells. This produces daughter cells with abnormal chromosome numbers.  Nondisjunction is common during anaphase I or anaphase II of meiosis.  Mutations in synaptonemal complex proteins that attach homologous chromosomes increase the chances of nondisjunction in anaphase I of meiosis I. In contrast, mutations in topoisomerases and condensins that hold sister...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zika virus infections of human stem cell-derived cerebral organoids reveal viral lineage-specific pathogenesis responses.

mBio·2026
Same author

Multisite Assessment of Methods for Cell Preservation Upstream of Single-Cell RNA Sequencing.

Journal of biomolecular techniques : JBT·2026
Same author

Evidence for G6PD variant classification from multiplexed functional assays.

Genome biology·2026
Same author

Deletion of low-essentiality, secretion-associated genes enhances recombinant protein production in Komagataella phaffii.

Microbial cell factories·2026
Same author

A cross-species rescue by mating method to interrogate gene essentiality across the <i>Saccharomyces</i> genus.

microPublication biology·2026
Same author

Beyond ERCs: exploring catastrophic forms of rDNA instability in aging yeast.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 8, 2026

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Identification of aneuploidy-tolerating mutations.

Eduardo M Torres1, Noah Dephoure, Amudha Panneerselvam

  • 1David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Cell
|September 21, 2010
PubMed
Summary

Cancer cells overcome growth disadvantages from aneuploidy through specific genetic mutations. A loss-of-function mutation in Ubp6, a deubiquitinating enzyme, improves growth in aneuploid yeast by regulating protein composition.

More Related Videos

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
08:46

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
08:46

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

Area of Science:

  • Cellular biology
  • Genetics
  • Cancer research

Background:

  • Aneuploidy, an abnormal chromosome number, typically hinders cell proliferation.
  • Despite this, aneuploidy is a hallmark of many cancers, which exhibit uncontrolled cell growth.
  • The mechanisms enabling cancer cells to tolerate aneuploidy remain unclear.

Purpose of the Study:

  • To investigate how cells adapt to and tolerate aneuploidy.
  • To identify genetic alterations that confer a proliferative advantage to aneuploid cells.
  • To understand the molecular basis of aneuploidy tolerance in cancer.

Main Methods:

  • Creation and analysis of aneuploid yeast strains with enhanced proliferative capacity.
  • Molecular characterization of identified aneuploid strains to pinpoint genetic alterations.
  • Assessing the impact of specific mutations on growth rates and intracellular protein composition.

Main Results:

  • Identified both strain-specific and shared genetic alterations in aneuploid yeast strains.
  • A loss-of-function mutation in the Ubp6 gene significantly improved growth rates in four distinct aneuploid strains.
  • This Ubp6 mutation attenuated aneuploidy-induced changes in intracellular protein composition.

Conclusions:

  • Aneuploidy-tolerating mutations exist and can improve the fitness of various aneuploidies.
  • The ubiquitin-proteasomal degradation pathway plays a crucial role in mitigating the negative effects of aneuploidy.
  • Understanding these mechanisms could offer new insights into cancer development and treatment.