Sample evaporation from pierceable cups: still an important source of analytical error
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Updated: Jun 8, 2026

VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders
Published on: August 3, 2016
Sofie Schouwers1, Eric Cuypers, Sabine Vervaet
1Laboratory for Clinical Chemistry and Toxicology, ZNA Stuivenberg, Antwerp, Belgium.
Background:
We illustrate the impact of sample evaporation on analytical results in laboratory practice and highlight preventive measures.
Methods:
Plasma (n=10) was analysed for glucose, Na(+), HCO(3)(-) and calcium on six different sample configurations at 5 time points within 2h.
Results:
With time glucose, Na(+) and calcium values increased and HCO(3)(-) values decreased in a clinically significant way.
Conclusions:
Analytical error due to evaporation may be significant, but can be reduced with optimal sample handling. A pierceable cover does not prevent loss of HCO(3)(-).
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