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Updated: Jun 8, 2026

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
Published on: April 22, 2022
Analytical performances of the Diazyme ADA assay on the Cobas® 6000 system
Hervé Delacour1, Christophe Sauvanet, Franck Ceppa
1Hôpital d'Instruction des Armées Bégin, Service de biochimie, Toxicologie, Pharmacologie Cliniques, 69, Saint Mandé, France. h_delacour@yahoo.fr
Objective:
To evaluate the analytical performance of the Diazyme ADA assay on the Cobas® 6000 system for pleural fluid samples analysis.
Design And Methods:
Imprecision, linearity, calibration curve stability, interference, and correlation studies were completed.
Results:
The Diazyme ADA assay demonstrated excellent precision (CV<4%) over the analytical measurement range (0.5-117 U/L). Bilirubin above 50 μmol/L and haemoglobin above 177 μmol/L interfered with the test, inducing a negative and a positive interference respectively. The Diazyme ADA assay correlated well with the Giusti method (r(2)=0.93) but exhibited a negative bias (~ -30%).
Conclusions:
The Diazyme ADA assay on the Cobas® 6000 system represents a rapid, accurate, precise and reliable method for determination of ADA activity in pleural fluid samples.
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