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Calcium mobilization in activated mast cells monitored by flow cytometric analysis
G L Rossi1, D J Young, S I Wasserman
1Department of Medicine, University of California, San Diego School of Medicine.
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|November 1, 1990
Abstract:
Changes in intracellular calcium levels induced in mast cells by either antigen or ionomycin were monitored using flow cytometry and the calcium binding dye indo-1. Both stimuli increased calcium levels in responsive cells by a similar amount, but not all cells examined were responsive to antigen. Antigen-induced increases in intracellular calcium levels could be completely blocked by the calcium antagonist TMB-8. Flow cytometry appears to be a useful method for monitoring mast cell calcium concentrations, as it provides the capability to study large numbers of individual cells.