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Ambroxol inhibits interleukin 1 and tumor necrosis factor production in human mononuclear cells
M Bianchi1, A Mantovani, A Erroi
1Laboratory of Immunology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Abstract:
We have studied the effect of Ambroxol on the production of Interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF) in human mononuclear cells (MNC). For this purpose MNC were cultured for 24 h in the presence of endotoxin and different doses of Ambroxol. The results indicate that Ambroxol markedly inhibited IL-1 and TNF production at doses of 10-100 microgram ml, without any apparent toxicity.
Insights
Ambroxol effectively reduces the production of Interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF) in human immune cells. This study shows Ambroxol
Area of Science:
- Immunology
- Pharmacology
Background:
- Interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF) are key inflammatory cytokines.
- Understanding modulators of cytokine production is crucial for inflammatory disease research.
Purpose of the Study:
- To investigate the impact of Ambroxol on IL-1 and TNF production.
- To assess the safety profile of Ambroxol in human mononuclear cells.
Main Methods:
- Human mononuclear cells (MNC) were cultured with endotoxin and varying doses of Ambroxol.
- Cytokine levels (IL-1 and TNF) were measured after 24 hours of incubation.
Main Results:
- Ambroxol significantly inhibited the production of IL-1 and TNF.
- Inhibition was observed at Ambroxol doses ranging from 10-100 microgram/ml.
- No apparent toxicity was detected in the treated cells.
Conclusions:
- Ambroxol demonstrates potent anti-inflammatory properties by suppressing key cytokine production.
- Ambroxol is a potential therapeutic agent for conditions involving elevated IL-1 and TNF.
- The drug exhibits a favorable safety profile at effective concentrations.