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Updated: Jun 8, 2026

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
Published on: May 2, 2025
Evanescent wave fluorescence biosensor combined with DNA bio-barcode assay for platelet genotyping
Marie Trévisan1, Manfred Schawaller, Gerald Quapil
1Université de Lyon, Institut des Nanotechnologies de Lyon, UMR 5270, site Ecole Centrale de Lyon, 36 avenue Guy de Collongue, 69134 Ecully Cedex, France.
Abstract:
An evanescent wave fluorescence biosensor was combined with a DNA bio-barcode assay to resolve problems met in detection of poor biologic samples. Human platelet antigen (HPA) genotyping was used as a demonstrator. Our bio-barcode assay was based on magnetic carboxylatex particles and non-magnetic carboxylatex particles, both functionalized with oligonucleotides. It was assessed for detecting 84mer synthetic oligonucleotides as targets. The assay allows to specifically detect single nucleotide polymorphism with a detection limit of 2 pM of target nucleic acids. The fluorescence detection is achieved in 150 s.
