Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FAIR in practice: minimum metadata schema for bioinformatics analytics by machines.

Journal of biomedical semantics·2026
Same author

Transcription-based identification of uncharacterized genes in the human immune response.

European journal of human genetics : EJHG·2026
Same author

KG-bench: benchmarking graph neural network algorithms for drug repurposing.

Bioinformatics (Oxford, England)·2026
Same author

Commitment to Myogenic Differentiation Significantly Aggravates the RNA Phenotype in Myotonic Dystrophy Type 1.

Neuropathology and applied neurobiology·2026
Same author

287th ENMC international workshop: Harmonization and federated analysis of myotonic dystrophy registries to model heterogeneous disease trajectories. Hoofddorp, the Netherlands, 28-30 March 2025.

Neuromuscular disorders : NMD·2025
Same author

The FAIR journey of a patient-driven registry: Reflections and practical solutions from the Duchenne Data Platform FAIRification experience.

Journal of neuromuscular diseases·2025

Related Experiment Video

Updated: Jun 8, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

mRNA degradation controls differentiation state-dependent differences in transcript and splice variant abundance.

Peter A C 't Hoen1, Michael Hirsch, Emile J de Meijer

  • 1Center for Human and Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands. p.a.c.hoen@lumc.nl

Nucleic Acids Research
|September 21, 2010
PubMed
Summary

Messenger RNA (mRNA) stability significantly impacts gene expression levels, varying with cell differentiation. Alternative splicing also influences mRNA degradation, affecting splice variant abundance.

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Related Experiment Videos

Last Updated: Jun 8, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Gene expression is typically measured as a static snapshot of messenger RNA (mRNA) levels.
  • Understanding mRNA dynamics, including synthesis and degradation, is crucial for developing effective gene expression control models.
  • Myogenic cell differentiation involves complex regulatory mechanisms influencing gene expression patterns.

Purpose of the Study:

  • To investigate mRNA stability in proliferating versus differentiated myogenic cells.
  • To determine the contribution of mRNA decay rates to differential gene expression.
  • To explore the relationship between alternative splicing and mRNA degradation.

Main Methods:

  • Utilized whole-genome exon arrays to analyze approximately 7000 mRNAs.
  • Quantified mRNA decay rates, including half-life measurements.
  • Compared mRNA stability profiles in proliferating and differentiated myogenic cell states.

Main Results:

  • Identified that mRNA stability is highly dependent on the differentiation status of myogenic cells.
  • Demonstrated that mRNA stability differences contribute significantly to variations in mRNA abundance.
  • Revealed a coupling between alternative splicing and mRNA degradation pathways.
  • Showed that transcript isoforms, even with shared 3'-untranslated regions (3'-UTRs), exhibit differential stability.
  • Observed distinct stability profiles for Itga7 splice variants in proliferating and differentiated cells.

Conclusions:

  • mRNA stability and degradation are critical regulatory mechanisms controlling differential gene expression.
  • Alternative splicing plays a significant role in determining the abundance of specific mRNA splice variants.
  • The findings challenge the notion that 3'-UTRs are the sole determinants of RNA stability, highlighting the role of internal exonic sequences.