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Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...

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A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
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MatrixDB, the extracellular matrix interaction database.

Emilie Chautard1, Marie Fatoux-Ardore, Lionel Ballut

  • 1Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS-Université Lyon 1, IFR 128 Biosciences Gerland-Lyon Sud, 7 passage du Vercors 69367, Lyon Cedex 07, France.

Nucleic Acids Research
|September 21, 2010
PubMed
Summary
This summary is machine-generated.

MatrixDB is a unique database detailing extracellular protein and polysaccharide interactions. It curates complex data, offering valuable resources for researchers studying extracellular matrices and their functions.

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Area of Science:

  • Biochemistry
  • Bioinformatics
  • Molecular Biology

Background:

  • Extracellular protein and polysaccharide interactions are crucial for cellular functions but are underrepresented in biological databases.
  • Existing databases lack comprehensive coverage of extracellular interactions, necessitating a specialized resource.

Purpose of the Study:

  • To introduce MatrixDB, a freely accessible database dedicated to cataloging extracellular protein and polysaccharide interactions.
  • To provide a centralized resource for researchers investigating the extracellular matrix and its associated molecular interactions.

Main Methods:

  • Curation of interaction data, considering the multimeric nature of extracellular proteins.
  • Adoption of Proteomics Standards Initiative-Molecular Interaction (PSI-MI) standards for data curation and exchange.
  • Integration of experimental data, literature curation, and data from other reputable databases like the Human Protein Reference Database.

Main Results:

  • MatrixDB offers a comprehensive collection of extracellular interactions, including those involving individual polypeptide chains and protein multimers.
  • The database incorporates data from diverse sources, ensuring a rich and varied dataset.
  • MatrixDB provides links to related resources for gene mutations, gene expression, and protein localization, enhancing its utility.

Conclusions:

  • MatrixDB serves as a valuable and unique resource for studying extracellular interactions, filling a critical gap in biological data repositories.
  • The database facilitates the construction and analysis of tissue- and disease-specific interaction networks.
  • MatrixDB supports research in cell biology, disease mechanisms, and drug discovery by providing integrated and accessible interaction data.