Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Mismatch Repair01:36

Mismatch Repair

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heparin Binds Lamprey Angiotensinogen and Promotes Thrombin Inhibition through a Template Mechanism.

The Journal of biological chemistry·2016
Same author

Repression of liver colorectal metastasis by the serpin Spn4A a naturally occurring inhibitor of the constitutive secretory proprotein convertases.

Oncotarget·2014
Same author

Origin of serpin-mediated regulation of coagulation and blood pressure.

PloS one·2014
Same author

Bioinformatic approaches for the identification of serpin genes with multiple reactive site loop coding exons.

Methods in enzymology·2011
Same author

An unexpected link between angiotensinogen and thrombin.

FEBS letters·2011
Same author

Multiple gains of spliceosomal introns in a superfamily of vertebrate protease inhibitor genes.

BMC evolutionary biology·2009

Related Experiment Video

Updated: Jun 8, 2026

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
13:10

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Published on: September 8, 2010

Intron creation and DNA repair.

Hermann Ragg1

  • 1Department of Biotechnology, University of Bielefeld, Germany. hr@zellkult.techfak.uni-bielefeld.de

Cellular and Molecular Life Sciences : CMLS
|September 21, 2010
PubMed
Summary

The origin of spliceosomal introns in eukaryotic genes is clarified. Novel introns form frequently, often through DNA repair mechanisms, challenging previous assumptions about intron creation.

Area of Science:

  • Molecular Genetics
  • Genomics
  • Evolutionary Biology

Background:

  • The origin and evolutionary mechanisms of spliceosomal introns in eukaryotic genes remain a significant, debated question in molecular genetics.
  • Intron gain, the process by which new introns are inserted into genes, was previously thought to be a rare event.

Purpose of the Study:

  • To review current understanding of intron gain mechanisms.
  • To present evidence supporting the DNA repair hypothesis as a primary driver of intron creation.
  • To discuss the implications of these findings on the structure of eukaryotic genes.

Main Methods:

  • Review of existing literature on intron gain and DNA repair mechanisms.
  • Analysis of recent discoveries regarding the frequency and patterns of novel intron formation.

More Related Videos

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Related Experiment Videos

Last Updated: Jun 8, 2026

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
13:10

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Published on: September 8, 2010

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

  • Synthesis of evidence to support a unified hypothesis for intron genesis.

    • Main Results:

    • The formation of new introns in eukaryotes, including vertebrates, is more common than previously believed.
    • Introns can be gained in parallel at nearby sites and repeatedly at the same genomic location.
    • Evidence supports DNA repair processes as a significant source for the creation of new introns.

    Conclusions:

    • The DNA repair hypothesis offers a comprehensive mechanistic framework for intron creation.
    • These findings reshape our understanding of the dynamic and mosaic structure of eukaryotic genomes.
    • The frequency of intron gain suggests a more active evolutionary process than previously recognized.