Related Experiment Videos
Mannose-6-phosphate isomerase polymorphism in baboon erythrocytes
J L VandeBerg1, M J Aivaliotis
1Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228-0147.
Biochemical Genetics
|October 1, 1990
Summary
Erythrocytic mannose-6-phosphate isomerase (MPI) is detectable in baboons, with four allelic isozymes found. The MPI*C allele is most frequent across all baboon subspecies studied.
Area of Science:
- Biochemistry
- Genetics
- Primate Studies
Background:
- Mannose-6-phosphate isomerase (MPI) was previously thought undetectable in mammalian erythrocytes.
- This study investigates the presence and genetic variability of MPI in baboons.
Purpose of the Study:
- To determine if erythrocytic MPI is detectable and analyzable in baboons.
- To characterize the genetic variation and allele frequencies of MPI in different baboon subspecies.
- To confirm the genetic basis of erythrocytic MPI.
Main Methods:
- Electrophoretic analysis of mannose-6-phosphate isomerase (MPI) in baboon erythrocytes.
- Survey of MPI phenotypes in 2656 baboons across five subspecies.
- Pedigree analysis to study segregation of allelic isozymes.
- Comparison of MPI phenotypes between erythrocytes and liver tissue.
Main Results:
- Sufficient erythrocytic MPI activity was detected for electrophoretic analysis in baboons.
- Four codominantly segregating allelic isozymes of MPI were identified.
- Significant differences in MPI gene frequencies were observed among five baboon subspecies.
- The MPI*C allele exhibited the highest frequency (0.830–1.000) across all subspecies.
- Identical MPI phenotypes in erythrocytes and liver indicate a shared genetic locus.
Conclusions:
- Erythrocytic MPI is detectable and genetically variable in baboons.
- The MPI gene locus is conserved between baboon erythrocytes and liver.
- MPI allele frequencies provide insights into genetic differentiation among baboon subspecies.