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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 8, 2026

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Published on: December 13, 2012

Dynamic proliferation assessment in flow cytometry.

Simone Diermeier-Daucher¹, Gero Brockhoff

¹Department of Gynaecology and Obstetrics, University of Regensburg, Regensburg, Germany.

Current Protocols in Cell Biology

|September 21, 2010

Summary

This study introduces 5-ethynyl-2'-deoxyuridine (EdU) as a superior alternative to 5-bromo-2'-deoxyuridine (BrdU) for dynamic cell proliferation assessment. EdU offers faster, simplified cell preparation and versatile applications in cell cycle analysis and live/dead cell discrimination.

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Last Updated: Jun 8, 2026

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Published on: December 13, 2012

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

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Published on: August 16, 2015

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Area of Science:

Cell Biology
Biochemistry
Molecular Biology

Background:

Flow cytometry is a powerful tool for analyzing cell cycle kinetics in vitro.
Traditional methods use thymidine analogs like 5-bromo-2'-deoxyuridine (BrdU) for labeling proliferating cells.
BrdU detection requires complex protocols and can be time-consuming.

Purpose of the Study:

To evaluate 5-ethynyl-2 -deoxyuridine (EdU) as an alternative to BrdU for dynamic proliferation assessment.
To explore EdU's utility in combination with live/dead cell discrimination.
To investigate EdU for continuous cell labeling in dynamic cell cycle analysis.

Main Methods:

Utilizing click chemistry for EdU detection, offering a simplified and rapid cell preparation.
Applying EdU pulse labeling for dynamic proliferation assessment.
Combining EdU labeling with live/dead cell discrimination.
Employing continuous EdU labeling with Hoechst fluorochrome quenching for cell cycle analysis.

Main Results:

EdU incorporation can be detected efficiently using click chemistry.
EdU labeling allows for simplified and faster cell preparation compared to BrdU.
EdU is suitable for dynamic proliferation assessment, live/dead cell discrimination, and cell cycle analysis.

Conclusions:

EdU serves as a versatile and efficient thymidine analog for dynamic proliferation studies.
Click chemistry-based detection of EdU offers significant advantages in speed and simplicity.
EdU expands the possibilities for high-resolution, single-cell based proliferation and cell cycle analyses.