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Food allergens profiling with an imaging surface plasmon resonance-based biosensor.

Sabina Rebe Raz¹, Hong Liu, Willem Norde

¹RIKILT-Institute of Food Safety, Wageningen UR, P.O. Box 230, 6700 AE Wageningen, The Netherlands. sabina.rebe@wur.nl

Analytical Chemistry

|September 22, 2010

Summary

A new imaging surface plasmon resonance (iSPR) method offers rapid, quantitative detection of multiple food allergens. This technology provides a complete allergen profile, enhancing food safety for consumers.

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Area of Science:

Food science and technology
Analytical chemistry
Immunotechnology

Background:

Food allergy is a significant public health issue, affecting a substantial portion of adults and infants.
Current methods for detecting major allergens in food are often labor-intensive, time-consuming, and costly.
Accurate and efficient allergen detection is crucial for consumer protection and regulatory compliance.

Purpose of the Study:

To develop and validate a rapid, quantitative, and multianalyte method for food allergen detection.
To utilize imaging surface plasmon resonance (iSPR) combined with antibody arrays for enhanced allergen profiling.
To assess the sensitivity and applicability of the iSPR method in real-world food products.

Main Methods:

Development of an iSPR-based assay utilizing antibody arrays for simultaneous detection of multiple food allergens.

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Application of the iSPR method to analyze commercially available food products, including cookies and dark chocolate.

Validation of iSPR results by comparing hazelnut content quantification with traditional enzyme-linked immunosorbent assay (ELISA).

Main Results:

The iSPR method enabled rapid and quantitative detection of food allergens with adequate sensitivity.
Analysis of food products demonstrated the technology's applicability in providing a complete allergen profile.
Quantification of hazelnut content using iSPR showed strong correlation with ELISA results, confirming accuracy.

Conclusions:

The developed iSPR technology offers a significant advancement for efficient and high-throughput food allergen analysis.
This method facilitates automated allergen detection, contributing to improved food safety and consumer protection.
The iSPR approach provides a promising alternative to conventional laborious methods for routine allergen monitoring.