Related Experiment Videos
Conditioned medium from mouse sarcoma 180 cells contains vascular endothelial growth factor
R A Rosenthal1, J F Megyesi, W J Henzel
1Department of Surgery, Children's Hospital, Boston, MA.
Growth Factors (Chur, Switzerland)
|January 1, 1990
Summary
Mouse sarcoma cells secrete a growth factor that stimulates endothelial cell proliferation. This factor is identified as vascular endothelial growth factor (VEGF), crucial for blood vessel formation.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Mouse sarcoma 180 cells release factors stimulating capillary endothelial cell growth.
- The secreted factor exhibits characteristics similar to vascular endothelial growth factor (VEGF).
Purpose of the Study:
- To identify and characterize the endothelial cell growth factor produced by mouse sarcoma 180 cells.
- To determine if this factor is related to vascular endothelial growth factor (VEGF).
Main Methods:
- Biochemical characterization of the secreted growth factor (heparin-binding, dithiothreitol-sensitive, endothelial cell-specific).
- Western blot analysis using an antibody against vascular permeability factor (VPF).
- Northern blot analysis using a cDNA probe for bovine VEGF.
Main Results:
- The sarcoma-derived growth factor shares properties with VEGF, including heparin-binding and endothelial cell specificity.
- Western blot analysis detected multiple VEGF-like proteins (approx. 23 kDa) using a VPF antibody.
- Northern blot confirmed the presence of VEGF mRNA in mouse sarcoma 180 cells.
Conclusions:
- The endothelial cell growth factor secreted by mouse sarcoma 180 cells is identified as vascular endothelial growth factor (VEGF).
- This finding highlights VEGF's role in tumor-associated angiogenesis.