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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

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Related Experiment Video

Updated: Jun 8, 2026

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

PsOr1, a potential target for RNA interference-based pest management.

Y Y Zhao¹, F Liu, G Yang

¹Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.

Insect Molecular Biology

|September 22, 2010

Summary

Researchers used RNA interference (RNAi) to silence the PsOr1 gene in the insect pest Phyllotreta striolata. This impaired the beetle

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Last Updated: Jun 8, 2026

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

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RNA Interference in the Egg Parasitoid, Trichogramma dendrolimi Matsumura

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Published on: November 21, 2023

Area of Science:

Entomology
Molecular Biology
Pest Management

Background:

Insect pests cause significant agricultural losses globally.
Existing control methods like insecticides face challenges such as resistance and environmental pollution.
Novel strategies are crucial for managing insect pests effectively.

Purpose of the Study:

To clone and characterize the PsOr1 gene from Phyllotreta striolata, a key agricultural pest.
To investigate the role of PsOr1 in insect olfaction and host-plant preference.
To evaluate the potential of RNA interference (RNAi) targeting PsOr1 for pest control.

Main Methods:

Cloning and characterization of the PsOr1 gene.
RNA interference (RNAi) using double-stranded RNA (dsRNA) targeting PsOr1.
Chemotactic behavioral assays to assess olfactory responses.
Reverse Transcription (RT)-PCR to confirm gene silencing.
Host-plant preference experiments.

Main Results:

PsOr1 was cloned and characterized, showing conservation with other insect odorant receptors.
RNAi-mediated silencing of PsOr1 significantly impaired the olfactory responses of P. striolata adults.
Down-regulation of PsOr1 mRNA levels was confirmed via RT-PCR.
Silencing PsOr1 disrupted host-plant preference for cruciferous vegetables.

Conclusions:

The PsOr1 gene plays a critical role in the olfaction and host-plant selection of Phyllotreta striolata.
Targeting PsOr1 using RNAi presents a promising, specific strategy for controlling this devastating insect pest.
This approach holds potential for broader application in managing diverse insect pests by disrupting their host-seeking behaviors.