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Published on: June 8, 2012
[Expression of microRNA-146a in lipopolysaccharide challenged alveolar macrophages in vitro]
Fen Liu1, Zhen-Guo Zeng, Cheng-Zhi Ding
1Intensive Care Unit, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.
Objective:
To investigate the expression of microRNA-146a (miRNA-146a) in NR8383 alveolar macrophages treated with lipopolysaccharides (LPS).
Methods:
NR8383 alveolar macrophages were divided into two groups: LPS treated group and phosphate buffer saline (PBS) control group, and they cultured for 6 hours. The production of tumor necrosis factor-α (TNF-α) in the supernatant of cells was determined with enzyme-linked immunosorbent assay (ELISA), and the expression of miRNA-146a of cells was detected by real-time polymerase chain reaction (PCR).
Results:
Compared with PBS control group, the TNF-α content (ng/L) in LPS treated group was significantly increased (650.26±40.53 vs. 6.23±1.76, P<0.01), and miRNA-146a in LPS treated group increased by about (5.33±0.81) folds (P<0.01).
Conclusion:
The expression of miRNA-146a was increased in LPS treated NR8383 cells, and miRNA-146a may be involved in the modulation inflammatory response of the NR8383 alveolar macrophage.
Insights
Lipopolysaccharides (LPS) significantly increased tumor necrosis factor-alpha (TNF-α) and microRNA-146a (miRNA-146a) expression in NR8383 alveolar macrophages, suggesting miRNA-146a
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Context:
- NR8383 alveolar macrophages are critical immune cells in the lung.
- Lipopolysaccharides (LPS) are potent inducers of inflammatory responses.
- MicroRNAs play regulatory roles in cellular processes, including inflammation.
Purpose:
- To investigate the effect of LPS on miRNA-146a expression in NR8383 cells.
- To determine the relationship between miRNA-146a and TNF-α production.
Summary:
- LPS treatment significantly upregulated both TNF-α and miRNA-146a expression in NR8383 cells.
- ELISA and real-time PCR confirmed a ~5.33-fold increase in miRNA-146a levels.
- TNF-α levels increased substantially in LPS-treated cells compared to controls.
Impact:
- This study highlights the role of miRNA-146a in the inflammatory response of alveolar macrophages.
- Findings suggest miRNA-146a as a potential target for modulating lung inflammation.
