Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

[Anti-tumor factor in insects' hemolymph].

Y Nakajima¹, S Natori

¹Faculty of Pharmaceutical Sciences, University of Tokyo.

|June 1, 1990

Summary

Researchers purified a Sarcophaga lectin involved in insect defense. This galactose-binding lectin showed cytotoxic effects on tumor cells with murine macrophages, indicating potential therapeutic applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Meeting report.

Mycotoxin research·2013

Same author

The impairing effects of chaetochromin D on mitochondrial respiration and structure.

Mycotoxin research·2013

Same author

Functional flexibility of the honey bee hypopharyngeal gland in a dequeened colony.

Zoological science·2008

Same author

Identification of a novel gene, Mblk-1, that encodes a putative transcription factor expressed preferentially in the large-type Kenyon cells of the honeybee brain.

Insect molecular biology·2002

Same author

Effects of angiotensin converting enzyme inhibitor and angiotensin II type 1 receptor blocker on cardiac dysfunction induced by isoproterenol in dogs.

Journal of cardiovascular pharmacology·2002

Same author

Targeted disruption of a pupal hemocyte protein of Sarcophaga by RNA interference.

European journal of biochemistry·2001

Area of Science:

Insect immunology
Biochemistry
Cell biology

Background:

Sarcophaga peregrina larvae possess a defense system involving hemolymph lectins.
Injury to the insect body wall triggers the release of these lectins.

Purpose of the Study:

To purify and characterize a lectin from Sarcophaga peregrina larval hemolymph.
To investigate the lectin's role in insect defense and its interaction with mammalian cells.
To explore the molecular mechanisms of fat body dissociation and recognition in Sarcophaga pupae.

Main Methods:

Purification of lectin from Sarcophaga peregrina larval hemolymph.
Assays to determine lectin's binding specificity (galactose residues).
Cytotoxicity assays using tumor cells and murine macrophages.
Establishment of an in vitro system using pupal hemocytes and fat body.

Main Results:

A galactose-binding lectin was successfully purified from injured Sarcophaga larvae.
The Sarcophaga lectin exhibited cytotoxic effects on tumor cells in the presence of murine macrophages.
Murine macrophages were found to possess Sarcophaga lectin binding proteins.
A novel membrane protein on pupal hemocytes was identified, potentially mediating fat body recognition.

Conclusions:

The purified Sarcophaga lectin plays a role in insect defense mechanisms.
The lectin demonstrates potential for anti-cancer applications via macrophage-mediated cytotoxicity.
A new membrane protein is implicated in the recognition processes during Sarcophaga pupal development.

Related Experiment Videos