[Establishment and characterization of human cancer cell line (NOC-S) derived from a progressive lung cancer]

The results of the transplantation out of a progressive lung cancer tissue with leukocytosis showed that tumors were grown in nude mice at about 70 days after direct transplantation. A tumor was removed from the nude mouse for attempt at establishment of cell line in vitro. The medium used for the cell culture was MEM synthetic culture medium (Gibco Inc.) supplemented with 10% new borne bovine serum. Subcultures were performed on 5 to 6 days basis at 1:2 split by the use of 0.25% trypsin solution. The morphology of obtained cells was a spindle-like shape and the cell grew in a monolayered sheet with about 32 to 36 hr of population-doubling time. The modal chromosome number of this cell line was 44 including one large submetacentric and one minute marker chromosome. Cell lines were tested for tumorigenicity with an inoculum of five million cells in nude mice. This cell lines produced tumors with high rate. The tumor has been maintained in nude mice through serial passages over a period of one year. The temperature sensitivity and the colony stimulating factor-producing of this cells will be discussed.