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Updated: Jun 8, 2026
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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
Published on: September 18, 2014
[Flow cytometric analysis for detecting mitochondrial permeability transition pore opening].
Fang Yan1, Xiao-dong Ma, Xue-mei Tian
1Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. yanfang@fimmu.com
Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|September 22, 2010
Summary
This study presents a novel flow cytometry method to detect mitochondrial permeability transition pore (PTP) opening. Resveratrol effectively induces PTP opening, which can be accurately measured by this technique.
Area of Science:
- Biochemistry
- Cell Biology
- Mitochondrial Research
Background:
- Mitochondrial permeability transition pore (PTP) opening is crucial in cell death.
- Accurate detection methods for PTP opening are essential for research.
Purpose of the Study:
- To introduce a novel flow cytometry method for detecting PTP opening.
- To utilize a resveratrol-induced PTP opening model for validation.
Main Methods:
- Rat liver mitochondria were isolated and stained with nonyl acridine orange.
- Mitochondrial membrane potential was assessed using TMRE labeling and flow cytometry.
- PTP opening was monitored via changes in mitochondrial side-scattering (SSC) using flow cytometry.
Main Results:
- Flow cytometry confirmed the purity of isolated mitochondria.
- Resveratrol treatment decreased mitochondrial fluorescence intensity and SSC, indicating PTP opening.
- Ciclosporin A successfully inhibited resveratrol-induced PTP opening.
Conclusions:
- Flow cytometry provides an accurate and convenient method for assessing mitochondrial membrane potential.
- This technique enables reliable detection of mitochondrial swelling and PTP opening.
