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Evaluation of Adair binding constants from experimental data obtained with an Imai cell. Statistical weighting
1Department of Pharmacology, University of Virginia Health Sciences Center, Charlottesville 22908.
Abstract:
One group of laboratories uses an 'even-weighted', or unweighted, nonlinear least-squares method for the analysis of experimental oxygen binding data obtained with an Imai oxygenation apparatus. Another group uses an 'end-weighted' nonlinear least-squares analysis. With end weighting each observation is assigned a statistical weight which is proportional to Y(1-Y), where Y is the fractional saturation. In this work we discuss statistical weighting functions as applied to the Imai oxygenation apparatus and then determined what are the best weighting factors for an actual series of published experiments. Based on these calculations, it is concluded that the 'best' weighting for the Imai oxygenation apparatus is a very small amount of end weighting. Furthermore, the amount of end weighting is so small that even weighting, or no weighting at all, is also appropriate.