Related Experiment Videos
Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions. III. Estimation of the protein
N N Kalnin1, I A Baikalov, Venyaminov SYu
1Institute of Protein Research, Academy of Sciences of the USSR, Puschino, Moscow Region.
Biopolymers
|January 1, 1990
Abstract:
Infrared spectra of 13 globular proteins have been obtained in the 1800-1480-cm-1 region for H2O solutions. A method for estimating protein secondary structure from the ir spectrum has been developed. The method can also be used for estimating polypeptide and fibrous protein conformation. For the globular and fibrous proteins and polypeptides analyzed, the correlation coefficients between the ir and x-ray estimates of ordered helix, disordered helix, ordered beta-structure, disordered beta-structure, turns, and remainder were 0.98, 0.80, 0.99, 0.87, 0.90, and 0.92 respectively.