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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Visualizing light-triggered release of molecules inside living cells.

Ryan Huschka1,2, Oara Neumann3,2, Aoune Barhoumi1,2

  • 1Department of Chemistry, University, 6100 Main Street, Houston, Texas 77005.

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Gold nanoparticles enable light-triggered release of DNA and associated molecules within cells. This controlled release of deoxyribonucleic acid (DNA) and dyes like DAPI shows promise for gene therapy and cellular studies.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Biology

Background:

  • Gold nanoparticle-based vectors offer potential for intracellular gene delivery.
  • Plasmon resonance of nanoparticles can be harnessed for light-triggered molecular release.
  • Host-guest complexation allows molecules to associate with DNA on nanoparticles.

Purpose of the Study:

  • To demonstrate intracellular light-triggered release of molecules associated with DNA on nanoshells.
  • To visualize the release process using a fluorescent molecule (DAPI).
  • To explore applications in cellular studies and targeted therapies.

Main Methods:

  • Utilized gold nanoshells functionalized with double-stranded DNA (dsDNA) and DAPI.
  • Irradiated nanoshell-dsDNA-DAPI complexes at the plasmon resonance wavelength.
  • Observed DAPI release and diffusion within living cells using fluorescence microscopy.

Main Results:

  • Light illumination successfully dehybridized DNA, releasing DAPI molecules intracellularly.
  • Released DAPI molecules diffused to the nucleus and associated with endogenous DNA.
  • Low laser power and short irradiation times maintained cell viability.

Conclusions:

  • Intracellular light-triggered co-release of nonbiological molecules with DNA is feasible using nanoshells.
  • This method provides a highly controlled mechanism for molecular delivery.
  • Potential applications include cellular process studies and intracellular targeted therapies.