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Related Experiment Video

Updated: Jun 8, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

EMG1 is essential for mouse pre-implantation embryo development.

Xiaoli Wu¹, Sumit Sandhu, Nehal Patel

¹Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada.

BMC Developmental Biology

|September 23, 2010

Summary

Essential for mitotic growth 1 (EMG1) is crucial for early mouse embryonic development. Complete loss of EMG1 function in mice causes pre-implantation lethality, highlighting its essential role in mammalian development and Bowen-Conradi syndrome.

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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

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Published on: April 29, 2011

Area of Science:

Developmental Biology
Molecular Genetics

Background:

Essential for mitotic growth 1 (EMG1) is a conserved nucleolar protein vital for ribosome biogenesis in yeast.
Mutations in human EMG1 cause Bowen-Conradi syndrome (BCS), a severe developmental disorder.

Purpose of the Study:

To investigate the role of EMG1 in mammalian development using a mouse model.
To understand how EMG1 deficiency may lead to Bowen-Conradi syndrome.

Main Methods:

Examined Emg1 expression during mouse embryonic development.
Generated and characterized mice with a null mutation for Emg1 using gene targeting.

Main Results:

Emg1 is broadly expressed in early development, with specific patterns later.
Loss of EMG1 function arrested embryonic development before the blastocyst stage.
Emg1-/- embryos showed defects in cell lineage specification and nucleologenesis; p53 loss did not rescue lethality.

Conclusions:

Emg1 is essential for mouse pre-implantation development, consistent with its role in yeast.
Findings support that partial EMG1 deficiency causes Bowen-Conradi syndrome.
Complete EMG1 deficiency is incompatible with live birth.